Calculate your Master Mix here: Number of samples (including serial dilutions): This simple, one-component reverse transcription kit uses a short 26-minute protocol. HotStarTaq Master Mix is a ready-to-use mixture of HotStarTaq DNA Polymerase, QIAGEN PCR Buffer, and dNTPs. Product specifications for individual components in Q5 Hot Start High-Fidelity 2X Master Mix are available separately. It contains thermostable Fast DNA polymerase, uracil-N-glycosylase (UNG), dNTPs with dUTP, ROX dye (passive reference), and optimized buffer components for maximum robustness and reproducibility. Alternative Product: Try TaqPath ProAmp Master Mix, our newest and highest performing master mix for genotyping and copy number variation for accurate results, even from samples containing PCR inhibitors.With TaqPath ProAmp Master Mix, weve taken the best of TaqMan Genotyping Master Mix and added additional capabilities for your genotyping and copy number variation Realtime PCR Master Mix is applicable in TaqMan assays or hybridization probe assays, in combination with each probe. Are you doing COVID-19 related research? As the PCR reaction proceeds, at each round of amplification SYBR Green dye binds to dsDNA as it polymerizes, resulting in an increase in the level of fluorescence at the end of Are you doing COVID-19 related research? By using a master mix, we add in all the required components with enough volume for all our reactions so we only need to pipette just once per reaction into each well. It contains thermostable Fast DNA polymerase, uracil-N-glycosylase (UNG), dNTPs with dUTP, ROX dye (passive reference), and optimized buffer components for maximum robustness and reproducibility. Contains 1 X 5 mL tube, sufficient for 500 20-L reactions (10 L Master Mix per reaction). Realtime PCR Master Mix is applicable in TaqMan assays or hybridization probe assays, in combination with each probe. HotStarTaq DNA Polymerase is supplied in an inactive state and has no polymerase activity at ambient temperatures. SARS-CoV-2 detection components. Gold-standard TaqMan chemistry: This kit is designed for use with TaqMan Advanced miRNA Assays for qPCR, which draw on Thermo Fisher Scientific's industry-leading bioinformatics assay design pipeline and validation system to help ensure high specificity and minimal cross-reactivity, even for closely-related miRNA families. Get superior nucleic acid quantitation with Applied Biosystems Power SYBR Green Master Mix.Power SYBR Master Mix offers superior sensitivity and reproducibility, detecting as few as two copies of a target gene over a broad range of template concentrations. Everything you need for SYBR Green dyebased PCR amplification and detection in a dNTPs with dUTP, ROX dye (passive reference), and optimized buffer components. Reference dye is included separately. Custom probes Master mixes & reagents GMP & OEM services. TaqMan Fast Advanced Master Mix delivers accurate quantification and dependability in less time on an array of qPCR instrument platforms. Buffer components and proprietary additives in the TaqMan Fast Virus 1-Step Master Mix have been optimized to handle RT-PCR inhibitors (Figure 2) to help ensure consistent performance even with these difficult samples, so that you can be more confident in your results. Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA). Rely on Roche qPCR assay solutions to generate reliable and consistent real-time PCR results, along with complete assay information to facilitate compliance with the MIQE guidelines. cDNA synthesis reactions are easy to assemblejust add RNA template and water. Coupled with user-supplied primer sets and template, PowerUp SYBR Green Master Mix is designed to amplifiy targ Popular Compatibility with most real-time qPCR instruments ROX passive reference dye, and optimized buffer components. Contains 1 5 mL vial. dNTPs with dUTP, ROX dye (passive reference), and optimized buffer components. SARS-CoV-2 detection components. Components. These components enable efficient cDNA synthesis at higher temperatures and in less time. Provides superior data reproducibility with reduced pipetting variability using a convenient master mix format containing all the essential components (except RNA). Coupled with user-supplied primer sets and template, PowerUp SYBR Green Master Mix is designed to amplifiy targ Popular Compatibility with most real-time qPCR instruments ROX passive reference dye, and optimized buffer components. HotStarTaq DNA Polymerase is supplied in an inactive state and has no polymerase activity at ambient temperatures. FSQ-301 200 reactions High efficient cDNA synthesis master mix for real-time PCR with gDNA remover. Stability testing using up to 15 freeze/thaw cycles has shown no negative effect on master mix performance. SYBR Green Master Mix Advantages. Functional genomics. CoralLoad dyes supplied in PCR Kits such as, e.g., Taq, HotStarTaq, and TopTaq DNA Polymerase and TopTaq Master Mix do not interfere with most downstream enzymatic applications. dNTPs with dUTP, ROX dye (passive reference), and optimized buffer components. Sufficient for 500 reactions of 20 L each. Our latest RUO kit, the Luna SARS-CoV-2 RT-qPCR Multiplex Assay Kit, enables high throughput workflows for real-time detection of SARS-CoV-2 nucleic acid using hydrolysis probes.For simple, visual assay results, the SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit includes a color-changing pH indicator for detection of SARS-CoV TaqMan Fast Advanced Master Mix has been designed to match TaqMan Fast Advanced Master Mix delivers accurate quantification and dependability in less time on an array of qPCR instrument platforms. It is supplied at a 2X concentration. Inclusion of ezDNase Enzyme further accelerates the RT-qPCR workflow through an extremely simplified genomic DNA removal step. The master mix formulation is supplied at 2X concentration and contains all PCR components required for amplification and quantitation of DNA except primers/probes and DNA template. This simple, one-component reverse transcription kit uses a short 26-minute protocol. Achieve quality performance under various experimental conditions with this thermostable probe-based qPCR master mix. These components enable efficient cDNA synthesis at higher temperatures and in less time. Use TaqMan Universal Master Mix II to amplify complementary DNA (cDNA) and DNA targets for a variety of applications, including quantitation and genotyping. FSQ-301 200 reactions High efficient cDNA synthesis master mix for real-time PCR with gDNA remover. Realtime PCR Master Mix is applicable in TaqMan assays or hybridization probe assays, in combination with each probe. dNTPs with dUTP, ROX dye (passive reference), and optimized buffer components. Store at -5 to -30C. PCR Master Mixes and Supermixes. A master mix usually contains a thermostable DNA polymerase, dNTPs, MgCl 2, and proprietary additives in a buffer optimized for PCR. Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA). Store at -5 to -30C. Achieve quality performance under various experimental conditions with this thermostable probe-based qPCR master mix. Our latest RUO kit, the Luna SARS-CoV-2 RT-qPCR Multiplex Assay Kit, enables high throughput workflows for real-time detection of SARS-CoV-2 nucleic acid using hydrolysis probes.For simple, visual assay results, the SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit includes a color-changing pH indicator for detection of SARS-CoV Get superior nucleic acid quantitation with Applied Biosystems Power SYBR Green Master Mix.Power SYBR Master Mix offers superior sensitivity and reproducibility, detecting as few as two copies of a target gene over a broad range of template concentrations. The master mix formulation is supplied at 2X concentration and contains all PCR components required for amplification and quantitation of DNA except primers and DNA template. Rely on Roche qPCR assay solutions to generate reliable and consistent real-time PCR results, along with complete assay information to facilitate compliance with the MIQE guidelines. Therefore, variability should be kept to a minimum whenever possible. Contains 1 X 5 mL tube, sufficient for 500 20-L reactions (10 L Master Mix per reaction). TaqMan Fast Advanced Master Mix has been designed to match TaqMan Fast Advanced Master Mix delivers accurate quantification and dependability in less time on an array of qPCR instrument platforms. Everything you need for SYBR Green dyebased PCR amplification and detection in a Are you doing COVID-19 related research? Calculate your Master Mix here: Number of samples (including serial dilutions): A PCR master mix is a premixed concentrated solution that has all of the components for a real-time PCR reaction that are not sample-specific. TaqMan Universal Master Mix II is a convenient mix of components (except primers, probes, template, and water) necessary to perform a real-time polymerase chain reaction (PCR). To minimize sample-handling time, look no further than this real-time RT-PCR kit. This simple, one-component reverse transcription kit uses a short 26-minute protocol. Use TaqMan Universal Master Mix II to amplify complementary DNA (cDNA) and DNA targets for a variety of applications, including quantitation and genotyping. SuperScript IV VILO (SSIV VILO) Master Mix is a reaction master mix designed for fast, sensitive, and reproducible cDNA synthesis in RT-qPCR applications. PrimeScript RT Master Mix (Perfect Real Time) is designed for fast, efficient reverse transcription prior to real-time RT-PCR (qPCR) with a streamlined workflow. The master mix formulation is supplied at 2X concentration and contains all PCR components required for amplification and quantitation of DNA except primers/probes and DNA template. Custom probes Master mixes & reagents GMP & OEM services. However, for reproducible results, purification of PCR products using the QIAquick or MinElute PCR Purification Kits prior to enzymatic manipulation is recommended. Product specifications for individual components in Q5 Hot Start High-Fidelity 2X Master Mix are available separately. Product specifications for individual components in Q5 Hot Start High-Fidelity 2X Master Mix are available separately. TaqMan Fast Advanced Master Mix has been designed to match TaqMan Fast Advanced Master Mix delivers accurate quantification and dependability in less time on an array of qPCR instrument platforms. Our latest RUO kit, the Luna SARS-CoV-2 RT-qPCR Multiplex Assay Kit, enables high throughput workflows for real-time detection of SARS-CoV-2 nucleic acid using hydrolysis probes. However, for reproducible results, purification of PCR products using the QIAquick or MinElute PCR Purification Kits prior to enzymatic manipulation is recommended. Research samples commonly assayed for viruses include blood, dirt, and tissues. Genomic DNA or cDNA of interest can be quantitated with Luna qPCR, and existing as well as commercial qPCR assay primer sequences can be used. A master mix usually contains a thermostable DNA polymerase, dNTPs, MgCl 2, and proprietary additives in a buffer optimized for PCR. Coupled with user-supplied primer sets and template, PowerUp SYBR Green Master Mix is designed to amplifiy targ Popular Compatibility with most real-time qPCR instruments ROX passive reference dye, and optimized buffer components. The master mix formulation is supplied at 2X concentration and contains all PCR components required for amplification and quantitation of DNA except primers/probes and DNA template. TaqMan Universal Master Mix II is a convenient mix of components (except primers, probes, template, and water) necessary to perform a real-time polymerase chain reaction (PCR). gDNA Remover: 10 L: 4x DN Master Mix: 440 L: 5x RT Master Mix II: 400 L: 5x RT Master Mix II no RT-Control: 40 L: Nuclease-free water: 1000 L 2: Gold-standard TaqMan chemistry: This kit is designed for use with TaqMan Advanced miRNA Assays for qPCR, which draw on Thermo Fisher Scientific's industry-leading bioinformatics assay design pipeline and validation system to help ensure high specificity and minimal cross-reactivity, even for closely-related miRNA families. PrimeScript RT Master Mix (Perfect Real Time) is designed for fast, efficient reverse transcription prior to real-time RT-PCR (qPCR) with a streamlined workflow. SYBR Green dye is a fluorescent double-stranded DNA (dsDNA)- binding dye that is used to track the progress of DNA amplification in real-time PCR experiments. To minimize sample-handling time, look no further than this real-time RT-PCR kit. Use TaqMan Universal Master Mix II to amplify complementary DNA (cDNA) and DNA targets for a variety of applications, including quantitation and genotyping. Realtime PCR Master Mix series is a Taq DNA polymerase-based 2 x master mix for real-time PCR, which contains all components, except for the primer and probe. PrimeTime Gene Expression Master Mix is a 2X solution containing a hot-start antibody, Taq polymerase, and other components needed for probe-based qPCR in two-step RT-PCR experiments. Store at -5 to -30C. Provides superior data reproducibility with reduced pipetting variability using a convenient master mix format containing all the essential components (except RNA). Therefore, variability should be kept to a minimum whenever possible. Research samples commonly assayed for viruses include blood, dirt, and tissues. The 2X mix contains SYBR Green 1 Dye, AmpliTaq Gold Fast DNA Polymerase LD, dNTPs with dUTP/dTTP blend, Passive Reference 1, and optimized buffer components. gDNA Remover: 10 L: 4x DN Master Mix: 440 L: 5x RT Master Mix II: 400 L: 5x RT Master Mix II no RT-Control: 40 L: Nuclease-free water: 1000 L 2: NGS products; PrimeTime Gene Expression Master Mix; rhAmp Genotyping Master Mix and Reporter Mixes; RNase H2 Enzyme; Functional genomics. Contains 1 5 mL vial. Reference dye is included separately. These components enable efficient cDNA synthesis at higher temperatures and in less time. To minimize sample-handling time, look no further than this real-time RT-PCR kit. SYBR Green dye is a fluorescent double-stranded DNA (dsDNA)- binding dye that is used to track the progress of DNA amplification in real-time PCR experiments. Research samples commonly assayed for viruses include blood, dirt, and tissues. HotStarTaq DNA Polymerase is supplied in an inactive state and has no polymerase activity at ambient temperatures. Buffer components and proprietary additives in the TaqMan Fast Virus 1-Step Master Mix have been optimized to handle RT-PCR inhibitors (Figure 2) to help ensure consistent performance even with these difficult samples, so that you can be more confident in your results. HotStarTaq DNA Polymerase, a modified form of Taq DNA Polymerase, provides high specificity in hot-start PCR.. HotStarTaq DNA Polymerase. CoralLoad dyes supplied in PCR Kits such as, e.g., Taq, HotStarTaq, and TopTaq DNA Polymerase and TopTaq Master Mix do not interfere with most downstream enzymatic applications. Contains 1 5 mL vial. The 2X mix contains SYBR Green 1 Dye, AmpliTaq Gold Fast DNA Polymerase LD, dNTPs with dUTP/dTTP blend, Passive Reference 1, and optimized buffer components. cDNA synthesis reactions are easy to assemblejust add RNA template and water. Functional genomics. Genomic DNA or cDNA of interest can be quantitated with Luna qPCR, and existing as well as commercial qPCR assay primer sequences can be used. The master mix formulation is supplied at 2X concentration and contains all PCR components required for amplification and quantitation of DNA except primers/probes and DNA template. TaqPath qPCR Master Mix, CG, is a 2X formulation designed for gene expression and miRNA analysis. PCR Master Mixes and Supermixes. dNTPs with dUTP, ROX dye (passive reference), and optimized buffer components. TaqPath qPCR Master Mix, CG, is a 2X formulation designed for gene expression and miRNA analysis. Reference dye is included separately. Functional genomics. PrimeTime Gene Expression Master Mix is a 2X solution containing a hot-start antibody, Taq polymerase, and other components needed for probe-based qPCR in two-step RT-PCR experiments. It is supplied at a 2X concentration. ReverTra Ace qPCR RT Master Mix with gDNA Remover Code No. Contains 1 X 5 mL tube, sufficient for 500 20-L reactions (10 L Master Mix per reaction). Custom SARS-CoV-2 qPCR panels; SARS-CoV-2 kits and controls; Luminex SARS-CoV-2 RT-PCR Assay; Viral research. Use the calculator below to estimate how much of each component you will need for your qPCR. (This master mix is also available without ezDNase.) Research samples commonly assayed for viruses include blood, dirt, and tissues. Inclusion of ezDNase Enzyme further accelerates the RT-qPCR workflow through an extremely simplified genomic DNA removal step. Achieve increased potency and better specificity in loss-of-function studies using short oligos. Are you doing COVID-19 related research? The master mix formulation is supplied at 2X concentration and contains all PCR components required for amplification and quantitation of DNA except primers and DNA template. Buffer components and proprietary additives in the TaqMan Fast Virus 1-Step Master Mix have been optimized to handle RT-PCR inhibitors (Figure 2) to help ensure consistent performance even with these difficult samples, so that you can be more confident in your results. Our latest RUO kit, the Luna SARS-CoV-2 RT-qPCR Multiplex Assay Kit, enables high throughput workflows for real-time detection of SARS-CoV-2 nucleic acid using hydrolysis probes.For simple, visual assay results, the SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit includes a color-changing pH indicator for detection of SARS-CoV NGS products; PrimeTime Gene Expression Master Mix; rhAmp Genotyping Master Mix and Reporter Mixes; RNase H2 Enzyme; Functional genomics. SYBR Green Master Mix Advantages. Are you doing COVID-19 related research? Alternative Product: Try TaqPath ProAmp Master Mix, our newest and highest performing master mix for genotyping and copy number variation for accurate results, even from samples containing PCR inhibitors.With TaqPath ProAmp Master Mix, weve taken the best of TaqMan Genotyping Master Mix and added additional capabilities for your genotyping and copy number variation However, for reproducible results, purification of PCR products using the QIAquick or MinElute PCR Purification Kits prior to enzymatic manipulation is recommended. Achieve quality performance under various experimental conditions with this thermostable probe-based qPCR master mix. A PCR master mix is a premixed concentrated solution that has all of the components for a real-time PCR reaction that are not sample-specific. cDNA synthesis reactions are easy to assemblejust add RNA template and water.